diff --git a/yt_dlp/extractor/_extractors.py b/yt_dlp/extractor/_extractors.py
index 967010826e..e7e5d3ff58 100644
--- a/yt_dlp/extractor/_extractors.py
+++ b/yt_dlp/extractor/_extractors.py
@@ -646,6 +646,7 @@ from .fc2 import (
     FC2LiveIE,
 )
 from .fczenit import FczenitIE
+from .fenomiostream import FenomioStreamIE
 from .fifa import FifaIE
 from .filmon import (
     FilmOnChannelIE,
diff --git a/yt_dlp/extractor/fenomiostream.py b/yt_dlp/extractor/fenomiostream.py
new file mode 100644
index 0000000000..e3f5e688f1
--- /dev/null
+++ b/yt_dlp/extractor/fenomiostream.py
@@ -0,0 +1,135 @@
+import re
+
+from .common import InfoExtractor
+from ..utils import (
+    clean_html,
+    compat_HTMLParseError,
+    extract_attributes,
+    get_element_by_attribute,
+    get_element_by_class,
+    get_element_html_by_class,
+    get_element_text_and_html_by_tag,
+    get_elements_by_class,
+    get_elements_html_by_class,
+    smuggle_url,
+    unsmuggle_url,
+)
+
+
+def get_elements_by_tag(tag, html):
+    while True:
+        try:
+            _, element = get_element_text_and_html_by_tag(tag, html)
+            yield element
+            html = html[html.index(element) + len(element):]
+        except compat_HTMLParseError:
+            return
+
+
+class FenomioStreamIE(InfoExtractor):
+    _FENOMIOSTREAM_INSTANCES = [
+        # CUNI
+        r'medicalmedia\.eu',
+        r'pilot\.cuni\.fenomio\.stream',
+        r'ua\.stream\.cuni\.cz',
+        r'media\.fhs\.cuni\.cz',
+        r'stream\.ims\.fsv\.cuni\.cz',
+        r'video\.czp\.cuni\.cz',
+        r'stream\.knihovna\.cuni\.cz',
+        r'media\.ftvs\.cuni\.cz',
+        r'stream\.cuni\.cz',
+        r'pedmedia\.cuni\.cz',
+        # AMU
+        r'media\.amu\.cz',
+        r'media\.test\.amu\.cz',
+    ]
+    _VALID_URL = r'^https://(?P<domain>(www\.)?({}))/(?P<lang>..)/media/(?P<id1>[0-9a-f]{{32}})(/(?P<id2>[0-9a-f]{{32}}))?'.format('|'.join(_FENOMIOSTREAM_INSTANCES))
+    _TESTS = [{
+        'url': 'https://stream.cuni.cz/en/media/c382f68514e24fa28d768f5c0997f757',
+        'md5': '2049d066e009461c996020b25af218bd',
+        'info_dict': {
+            'id': 'c382f68514e24fa28d768f5c0997f757',
+            'title': 'S03/E06 - Searching For Open Access Works',
+            'creators': ['Milan Janíček', 'Zuza Wiorogórska'],
+            'ext': 'mp4',
+            'description': 'This session is a workshop whose goal is to help you doing your literature search in an open environment. We will present different search tools and show you how to use them. This training include practice time to allow you to search open access literature in your field.',
+            'tags': ['open access', 'publications'],
+            'license': 'CC BY 4.0',
+            'thumbnail': 'https://stream.cuni.cz/shared/mediasnapshots/c3/c382f68514e24fa28d768f5c0997f757.jpg',
+        },
+    }, {
+        'url': 'https://stream.knihovna.cuni.cz/cs/media/97c4c1f43ac946bf9fed1fb166e0ae6d',
+        'md5': 'b4d3bd95926e86e1e9115675b9bd4c0c',
+        'info_dict': {
+            'id': '97c4c1f43ac946bf9fed1fb166e0ae6d',
+            'ext': 'mp4',
+            'tags': [],
+            'creators': ['Jaroslav Pěnička'],
+            'title': 'Jak nahrát video',
+            'description': 'Jak nahrát video',
+            'thumbnail': 'https://stream.knihovna.cuni.cz/shared/mediasnapshots/97/97c4c1f43ac946bf9fed1fb166e0ae6d.jpg',
+        },
+    }]
+
+    def _real_extract(self, url):
+        url, smuggled_data = unsmuggle_url(url)
+
+        match = self._match_valid_url(url)
+        domain = match.group('domain')
+        lang = match.group('lang')
+        id1 = match.group('id1')
+        id2 = match.group('id2')
+        webpage = self._download_webpage(url, id1)
+
+        info_container = get_element_by_class('video-description-container', webpage)
+        if not info_container:
+            alert = get_element_by_class('alert-danger', webpage) or ''
+            alert = get_element_by_class('m-0', alert) or 'Unknown error'
+            alert = clean_html(alert)
+            self.raise_login_required(alert)
+
+        info = get_element_by_attribute('data-bind', 'visible: expandDescription()', info_container) or ''
+        info_ps = list(get_elements_by_tag('p', info)) + [''] * 9
+
+        title = self._html_search_regex(r'<h3>(.+)</h3>', info_container, 'title', flags=re.DOTALL, fatal=False)
+        creators = [x.strip() for x in clean_html(info_ps[0]).split(';')]
+        description = clean_html(info_ps[1])
+        license_name = clean_html(info_ps[2]) or None
+        tags = get_elements_by_class('tag', info)
+
+        source_url = self._html_search_regex([r'<source[^>]+src="([^"]+)"', r'self.videoUrl *= *\'([^\']+)\''], webpage, 'source', fatal=True)
+
+        playlist_info = get_element_html_by_class('video-playlist', webpage)
+        video_id = id1
+        if playlist_info:
+            playlist_title = self._html_search_regex(r'<h4>.+Playlist ([^<]+)</h4>', playlist_info, 'playlist title', fatal=False)
+            playlist_id = id1
+            video_id = id2
+            if self._yes_playlist(playlist_id, video_id, smuggled_data=smuggled_data, playlist_label=playlist_title, video_label=title):
+                return {
+                    '_type': 'playlist',
+                    'id': playlist_id,
+                    'title': playlist_title,
+                    'entries': [
+                        {
+                            '_type': 'url',
+                            'title': clean_html(playlist_item),
+                            'url': smuggle_url('https://{}/{}{}'.format(domain, lang, extract_attributes(playlist_item)['href']), {'force_noplaylist': True}),
+                            'ie_key': self.ie_key(),
+                        }
+                        for playlist_item in get_elements_html_by_class('playlist-item', playlist_info)
+                    ],
+                }
+        formats, subtitles = self._extract_m3u8_formats_and_subtitles(source_url, video_id)
+
+        return {
+            'formats': formats,
+            'subtitles': subtitles,
+            'id': video_id,
+            'title': title,
+            'creators': creators,
+            'description': description,
+            'tags': tags,
+            'license': license_name,
+            'thumbnail': f'https://{domain}/shared/mediasnapshots/{video_id[:2]}/{video_id}.jpg',
+        }