2012-09-12 20:53:10 +02:00
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Primer3 is a widely used program for designing PCR primers (PCR =
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"Polymerase Chain Reaction"). PCR is an essential and ubiquitous tool
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in genetics and molecular biology. Primer3 can design hybridization
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probes and sequencing primers.
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PCR is used for many different goals. Consequently, primer3 has many
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different input parameters that you control and that tell primer3
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2011-09-03 03:19:20 +02:00
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exactly what characteristics make good primers for your goals.
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For details and citation:
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2012-09-12 20:53:10 +02:00
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Steve Rozen and Helen J. Skaletsky (2000) Primer3 on the WWW for
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general users and for biologist programmers. In: Krawetz S, Misener S
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(eds) Bioinformatics Methods and Protocols: Methods in Molecular
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2011-09-03 03:19:20 +02:00
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Biology. Humana Press, Totowa, NJ, pp 365-386
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